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KMID : 0380020190340020107
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 2 p.107 ~ p.113
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Analysis of Quadruple Coating for Improvement in Improving Gastrointestinal Stability and Survivability of Probiotics
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Kim Min-Cheol
Moon Jin-Seok
Kim Young-Hoo
Kim Min-Goo
Kim Tae-Yoon
Park Ha-Young
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Abstract
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Probiotics exert multiple beneficial bioactive effects on the human body, including activation of gastrointestinal motility and suppression of harmful bacteria. However, the bioactive properties of probiotics are often lost after their ingestion due to chemical barriers in the gastrointestinal system, such as gastric and bile acids. In this study, we aimed to develop a quadruple coating technique for probiotics to prevent probiotics from being killed by external factors. Candidate coating mechanisms appropriate for the features of each coating stage were selected. The coating mechanism that led to the highest survival rate was chosen by quantifying the bacteria that survived after freeze-drying following coating. A quadruple coating technique was developed. Water-soluble polymer, hyaluronic acid, porous particles, and protein were applied at stages 1, 2, 3, and 4, respectively. Quadruple-coated probiotics - Lactobacillus acidophilus IDCC3302 demonstrated superior acid and bile tolerance with lower death rate at low and high temperatures compared with noncoated, single-coated, double-coated, and triple-coated probiotics. In conclusion, the quadruple-coating technique developed in this study can be applied for the production of probiotics while preserving the bioactive functions of the probiotics.
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KEYWORD
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Lactobacillus acidophilus IDCC3302, quadruple coating technology, gQlab, probiotics
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